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KMID : 0545120120220020166
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Journal of Microbiology and Biotechnology
2012 Volume.22 No. 2 p.166 ~ p.169
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Characterization of Stress Responses of Heavy Metal and Metalloid Inducible Promoters in Synechocystis PCC6803
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Barbara Blasi
Loredana Peca
Imre Vass
Peter B. Kos
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Abstract
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In several biotechnological applications of living bacterial cells with inducible gene expression systems, the extent of overexpression and the specificity to the inducer are key elements. In the present study, we established the concentration ranges of Zn2+, Ni2+, Co2+, AsO2 -, and Cd2+ ions that caused significant activation of the respective promoters of Synechocystis sp. without concomitant unspecific stress responses. The low expression levels can be increased up to 10-100-fold upon treatments with Cd2+, AsO2 -, Zn2+, and Co2+ ions and up to 800-fold upon Ni2+ treatment. These results facilitate the development of conditional gene expression systems in cyanobacteria.
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KEYWORD
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cyanobacterial gene expression, heavy metal toxicity, specificity of promoters, Synechocystis PCC6803, inducible promoters
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